FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2233_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2233_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044571
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA185500.9072807938682491No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA146230.7152111616568952No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA119820.5860398098182944No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT103690.5071479542652224No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG102920.5033818830453919No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG90650.4433692936073142No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA83890.4103061228981532No Hit
GACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATAAGAAATC80580.39411690765446644No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG77830.380666653297929No Hit
GACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATA77740.3802264631553514No Hit
GCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC76750.37538437158699794No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA70710.34584272201845767No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA68130.3332239379312335No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA67330.3293111366638771No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG66540.3254472454123628No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA63590.3110187907389863No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT62470.30554086896468746No Hit
GAGCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTAT60650.2966392460814518No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT58990.2885201834516874No Hit
CCCAACTGGTTGTTCAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATT55090.26944527727332535No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAAG54840.26822252687727643No Hit
AGTCTGTTCAGATGTCTGTTTCTTTAGAATTCAATCTTAGTAGGTTGTAT53550.26191313483366435No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC53050.2594676340415667No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA51310.25095729128506666No Hit
GGATCATTCCTTCCTCTTGTTTGTCTGGCAGAGTTGGAGAAGGATTGGTA51150.2501747310315954No Hit
GTAACAGGTTGTCTGAGGTTGATGTTTCTGTTGTGATCTTCTGTCTGGAT49680.24298495870282816No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT47170.23070854472649763No Hit
CCATCCAAGTAAGGGGTTGTCTGAGGCTGATGTTTCCATTGTGATCTTCT46740.2286054140452936No Hit
GACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGCTTAAATAAGAAATC46170.2258175431423022No Hit
AGAACAATATCAGATCAACAAAGAGCCGGTTCTTTGAAAAGAACAAAGCT44820.2192146910036384No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC43140.21099780834219012No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA42810.20938377781940565No Hit
GTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATG40580.19847684428664988No Hit
AGATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTAT39870.19500423316187113No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG39570.1935369326866125No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTACCCTTTTCTTTTGGATTA39030.190895791831147No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT37610.18395056958158948No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC36600.1790106579815521No Hit
TATGCAAAGGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGC35370.17299472603299176No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA35270.17250562587457224No Hit
GGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAGGGGTTGTCTG34960.17098941538347165No Hit
TCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTAT34800.1702068551300004No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT33740.16502239345075326No Hit
GCGTTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC33730.1649734834349113No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG33170.16223452254776186No Hit
GACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGCTTAAATA33140.16208779250023597No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT33110.16194106245271012No Hit
GATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTAT32780.16032703192992565No Hit
TGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATAAGAAAT32490.15890864147050898No Hit
ACACCAACAAGAATAACAGATCAACAAAGAGCTGGTTCTTTGAAAAGAAC31980.15641423066256932No Hit
AGTCTGTTCATATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT31830.15568058042494No Hit
GTAAGGGGTTGTCTGAGGTTGATGTTTCTGTTGTGATCTTCTGTCTGGAT31360.15338180968036816No Hit
GTTTTCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA30320.14829516803280493No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT30090.14717023766843998No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACTCTCCATCCAA29540.1444801867971325No Hit
GCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC29470.14413781668623882No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA28280.13831752480104628No Hit
ATTTCATCTCTCTACTGGTCATTAGTCTGTTCAGATGTCTTTTTCTTTAG28100.13743714451589112No Hit
CTTGGCAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTATC28020.13704586438915548No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA27900.13645894419905202No Hit
TGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTG27840.13616548410400028No Hit
CTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTG27830.13611657408815833No Hit
GATTCAAAGAAAGAGGACTCATCATCAGAAATGAGAGAGATGTTCCCTGA27550.13474709364458365No Hit
ACACCAACAAGAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAAC27480.13440472353368996No Hit
AGAACAATATCAGATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCT26770.1309321124089112No Hit
GAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA26280.12853552163265544No Hit
ACCACTGATATGCAAAGGCTCTTAAGAATGTACTATGAACAATTACACCC26040.12736168125244857No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA25900.1266769410306612No Hit
ATGACCAGTAGAGAGATTAAATAAGAAATCAAAAACTTCCAACAAGCAAA25250.12349779000093418No Hit
GGGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGT25230.12339996996925026No Hit
GTACAATTATCCAACATTTATAAATATTTACTGCCCCAGCATGGAGCAAC25040.12247067966825315No Hit
CATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATAAG24860.12159029938309798No Hit
GCTGACAGAGTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAAA24850.12154138936725603No Hit
GTACCCTTTTCTTTTGGATTATCCAGCTTGTTGGGGTGTAATTGTTCATA24350.11909588857515832No Hit
GTAGAATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23210.11352014676917553No Hit
GTTGCATCATTTTACTGCATTAATACCATTATCAGTTGTAGTGCCCTTCA23060.11278649653154622No Hit
GAGCATTCCTTCCTCTTGTTTGTCTGGCAGAGTTGGAGAAGGATTGGTAT23040.11268867649986232No Hit
GCATTCCTTCCTCTTGTTTGTCTGGCAGAGTTGGAGAAGGATTGGTATTC22940.11219957634144279No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA22310.10911824534339967No Hit
GAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACCAAATTCTAC22080.10799331497903472No Hit
GGTTATATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTATCCAGCTTG22050.10784658493150885No Hit
GCATCGTTTTACTGCATTAATTCCATTATCAGTTGTAGTGCCCTTCATTG22030.10774876489982495No Hit
GGCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAAT22000.10760203485229909No Hit
GATTCGTAGAATTCACAGGTGAAACCATGGGGTCCTGGACTTTTGCTTGT21880.10701511466219564No Hit
GAGGCTGATGTTTCCGTTGTGATCTTCTGTCTGGATGGGGGATCATTGAG21510.10520544407604333No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT21460.10496089399683356No Hit
GCATCATTTTACTGCATTAATACCATTATCAGTTGTAGTGCCCTTCATTG21260.10398269367999449No Hit
ACCTACTAAGATTGAATTCTAAAGAAACAGACATCTGACTGTTCAGCTGT20940.10241757317305195No Hit
TGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGCTTAAATAAGAAAT20900.10222193310968414No Hit
GGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAAT20490.10021662246016401No Hit
GATACATATTTTTTAAAAAACACCAACAAGAATAACAGATCAGCAAAGAG20480.10016771244432206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATAT14450.088.316494
ACATGGT30500.086.7592245
CAGTGCA53650.086.322972
TATCAGA14800.084.720828
AGTGCAG54800.084.579693
TGCAGCA36650.084.464955
AGTCCGC4550.084.42484145
CAGCAAC36700.084.329257
GTGCAGC55000.083.979594
GTTGACA31650.083.7789841
GCAGTGC56400.082.3071751
GCTACTA19250.081.325899
ATCAGAT15950.079.066849
ATCATTC40050.078.964123
GCCGACC1200.078.51776145
GACATGG33550.078.453284
CAGCTAC19850.078.139327
CTTATAT20950.078.0215151
CATGGTC34550.076.787746
AGCTACT20500.076.366998
Produced by FastQC (version 0.11.5)