FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2233_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2233_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605394
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC40330.2512155894440866No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG27950.1741005634753836No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA27410.17073690321503632No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG27100.16880591306557768No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT25460.15859035227489326No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT23330.1453225812479678No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA21990.13697572060192076No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT21340.13292687028853978No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG21330.13286458028371853No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA21220.1321793902306848No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21110.1314942001776511No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC20870.1299992400619412No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA20680.1288157299703375No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG19920.12408168960392277No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT19830.12352107956053156No Hit
GAAGAAAGTTTATAGTGATACAGGCCTAACTCAAGAAACAAGAAACATCT19580.1219638294400004No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA19150.11928535923268681No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG18120.11286948873609844No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT18090.11268261872163468No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA16930.10545697816237011No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA16830.10483407811415764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1900.053.410843145
CGAAGCG553.2206482E-452.7237665
ACATGGT19050.049.0912175
CAGTGCA10900.048.6048052
GTTGACA19500.048.0646481
GCGTTAA2650.046.500549
GTACTGC6250.046.39984
TATAGAC3300.041.78522
TGCGTTA2950.041.771678
GACATGG22650.041.2912144
TATTGGG8050.040.5694852
CCGAGGT3250.040.153674
TACTGCG3100.039.7554175
GGTCCTA31350.039.537899
AGTGCAG13650.039.321013
GACGACT1303.700352E-739.0382924
GGTTATA5100.038.4648671
GTATTAT17950.038.048191
CTGCCGC6950.037.546776145
ATTGTAC8150.037.442281
Produced by FastQC (version 0.11.5)