FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372124
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16710.12178199637933598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2002.2009772E-1036.312491
GTCGAAG2002.237357E-1036.2595333
TTACGTG2103.7471182E-1034.5530432
AGTCGAA2154.820322E-1033.7494852
GCTACTA2800.033.6548429
GGTGCGA1301.657205E-533.4557048
GTTGACA11600.031.3038671
CAGTGCA9000.030.6370322
TATACGA950.004726878430.5276684
AAGTCGA2451.9372237E-929.6428451
TAGTCGG2553.0304363E-928.4264138
GCGAAAG3453.6379788E-1227.3419742
GCCGACC1606.838075E-527.182758145
GAATCGC1358.0033275E-426.8981381
ACTCCGA4350.026.7126351
CCGAGGT4350.026.6678474
TATGGAC5450.026.6047425
ACATGGT13650.026.5560175
GTAATGC4400.026.4090791
CCGACTA4400.026.3648034
Produced by FastQC (version 0.11.5)