FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384230
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15570.11248130729719771No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG803.739159E-545.304787145
GATATAT5650.028.2896631
ACTCCGA4550.027.1450771
CCGAGGT4450.026.0658054
GTTACGT2258.945717E-725.832181
TTACGTG2001.0556552E-525.4064752
GTATACC2001.0612963E-525.3890253
GTATTAG3801.2732926E-1124.8549731
GTATTAT15450.023.9825421
ATCTATG6450.023.6544651
CCCGTAA1600.00214907522.7040621
GCCGACC2252.6559821E-522.55172145
CGAGGTG5150.022.522885
TTAGGTA4205.2750693E-1122.4390894
CTGCCGC4557.2759576E-1222.303898145
CGAAAGC4900.022.2214072
GTTGACA8750.021.5883221
GCGAAAG5051.8189894E-1221.58011
TATACTG7100.021.4423375
GTCTAGG4201.2369128E-920.7581
Produced by FastQC (version 0.11.5)