FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410196
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT25840.18323694011328923No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC22880.16224695006935205No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG22100.15671580404426053No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT21140.14990823970568629No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA19110.1355130776147429No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG17950.1272872707056324No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG17200.1219688610661213No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA15880.11260846010058177No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT15880.11260846010058177No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT15190.10771552323223155No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC15030.10658092917580252No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA14410.10218437720714No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG14300.10140434379334504No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC14190.10062431037955008No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC300.001919845272.652421
GTTACGT2450.062.2735061
TTACGTG2500.060.978192
AGTCGAA1500.053.2349322
TACGGTA553.220493E-452.723835
AAGTCGA1650.048.4349481
CAGTGCA10850.048.172372
CGAAGCG951.9223135E-645.786485
GTCGAAG1751.8189894E-1245.5927053
ACGTGTT3350.045.4496274
CCGACTA5300.045.1433684
GCTACTA3400.040.5077489
AGTGCAG12750.040.3913963
GTTATGT8700.040.0840951
ACATGGT14700.039.9463735
GTTGACA14950.039.3635181
GTGCAGC13100.039.295514
TATGGAC4800.039.268275
GGTTATA4350.038.4139251
GCCGACC1354.980011E-737.58614145
Produced by FastQC (version 0.11.5)