FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641252
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17700.1078444991993917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1406.5222775E-736.314051
AGCGAAA4800.031.7747921
ACGACTC3200.031.7167035
GCCGACC2807.2759576E-1231.06469145
AGACGAC3050.030.9090633
CGACTCC3350.030.292866
GCGAAAG5600.029.805742
GCTATAT2303.4311597E-828.4196911
CTAGTAC1555.4935397E-528.0712593
AACCGAT2156.076807E-727.0029772
AAGACGA3503.6379788E-1226.9547582
CGAAAGC5700.026.7169443
GACGACT3607.2759576E-1226.1828564
GAGTGCG2259.1307265E-725.7721887
CCGAGGT4850.025.4146024
CGGAACG2001.0678057E-525.36957
CTGCCGC7450.025.296526145
TAGTACC7900.023.8628564
TATAACG1851.8321637E-423.5119955
TATAGAC5350.023.0597882
Produced by FastQC (version 0.11.5)