FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233794
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT21520.1744213377597881No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT16050.1300865460522583No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC14620.11849628057844341No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG13930.11290377486030893No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT13670.11079645386506987No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13160.1066628626821009No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA12480.10115140777147563No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA406.669409E-572.48769145
GCTACTA2300.047.2745869
TTACGTG2000.047.182022
GTTACGT2100.044.9699331
CCGACTA4000.038.066844
GACTTAA5300.037.0071831
GACGACT1458.6928594E-735.003994
TATACTG5750.034.0446055
AGACGAC1551.4612906E-632.7536353
ACGTGTT2900.032.5037044
CAGTGCA7400.032.37022
GTTGACA10550.030.2969131
GATAGCG4500.028.99508145
GATATGT6350.028.5998941
ACATGGT10800.028.1953755
TACGTGT3351.8189894E-1228.1443823
CCACCGA5500.027.7366981
TATAACG1050.007707897627.6199595
GTATATC3204.1836756E-1127.24141
GTATTAT20050.027.1734661
Produced by FastQC (version 0.11.5)