FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756428
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG19620.1117039810342354No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17850.10162671057396035No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCA450.009643748.329076
CCGAGGT5550.040.4976924
CGACGAA750.001484334838.66326145
GTTACGT2300.037.9144941
ACGCGCC1157.1515133E-637.8216748
CTACGAG1354.9621667E-737.6042673
GTTATGT10650.035.48181
AGTCGAA1956.9303496E-933.4948232
ACTCCGA6300.033.451021
CGCGCCA1352.1448292E-532.2184649
CGAGGTG6600.031.8541585
AGCGAAA5050.031.6579781
TATACTG6900.031.5198575
TTACGTG3000.031.4479182
GGTTATA4500.030.6826571
GCTACTA2859.094947E-1230.5227579
AAGTCGA2151.7713319E-830.419771
GTTGACA17500.030.3135431
CGATACA1204.061381E-430.205671145
AACTACG1000.00600678429.067781
Produced by FastQC (version 0.11.5)