FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816124
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA216431.1917137816580805No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC177150.9754289905314835No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG176660.9727309368743544No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG144440.7953201433382302No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT96340.5304703863833087No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG88570.4876869641059751No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA84450.4650012884582771No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT81760.4501895245038335No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG77890.4288804068444666No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA76340.4203457473168131No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA76110.4190793139675485No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG72730.4004682499653108No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA70990.3908874063665256No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT67770.3731573394768199No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG63160.34777361017199265No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG54540.3003098907343331No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA53970.297171338520938No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAAACACTAGCCAAAGGG52110.28692974708775393No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA51080.2812583281758294No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA50680.2790558353944995No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG46840.2579119046937324No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA44810.24673425382848307No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC44310.24398113785182068No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC41360.22773775358951262No Hit
TCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGC40580.2234428926659193No Hit
CATCAATACCAGAAGTGCTGCACTCTATTCGAGAAATTCTTCCCTAGCAG39620.2181569099907275No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG39030.21490823313826588No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG39020.21485317081873265No Hit
TTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTA38410.21149436932720456No Hit
CTACTAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCA37870.20852100407240917No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGC37630.2071995084036112No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT37590.2069792591254782No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC37180.20472170402461506No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATACCAGAAGTGCTG36910.20323502139721739No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA36610.20158315181121994No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT35380.19481048650863048No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG35320.194480112591431No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT34990.1926630560468338No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG34110.187817571927908No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA34060.18754226033024177No Hit
GGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCT33250.1830822124480487No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG33120.1823664022941165No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA32500.17895253848305512No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG31840.17531842539386078No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA31570.17383174276646307No Hit
ACAAACACAGAGACCGGAGCATGCACAAACAGATTGTGTATTGGAAGCAA31540.17366655580786333No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA30160.16606795571227514No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG29650.1632597774160795No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG29610.16303952813794653No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA29550.16270915422074703No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA29480.1623237179840143No Hit
CTTCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGG29220.16089209767614987No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA29220.16089209767614987No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT29120.1603414744808174No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG28470.15676242371115628No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT28340.15604661355722407No Hit
GGTTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG28320.15593648891815756No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG28310.1558814265986243No Hit
GATCTTAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA28290.15577130195955782No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA27910.15367893381729442No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA27860.15340362221962817No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA27850.15334855990009494No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG27300.1503201323257663No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG27110.14927394825463458No Hit
GCTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCTAGTGTTGAGAATG26810.14762207866863716No Hit
ACAAAACACTAGCCAAAGGGGAATTCTTGAGGATGAACAAATGTACCAGA26810.14762207866863716No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA26190.1442082148575758No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC26120.14382277862084306No Hit
TCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTA25830.1422259713543789No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG25720.14162028583951317No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT25610.14101460032464744No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC25350.139582980016783No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT25220.13886716986285078No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA25050.13793111043078557No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT24820.13666467708152089No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG24650.13572861764945565No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA24610.13550836837132266No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG24530.13506786981505667No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT24320.1339115611048585No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC24050.1324248784774608No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA23990.1320945045602613No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG23940.13181919296259506No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC23720.13060782193286363No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT23720.13060782193286363No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA23620.13005719873753113No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG23620.13005719873753113No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA23500.12939645090313218No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT23170.127579394358535No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG23110.1272490204413355No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC23030.12680852188506953No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT22830.12570727549440455No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA22630.12460602910373962No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG22570.12427565518654013No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC22420.1234497203935414No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT22370.12317440879587517No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG22270.1226237856005427No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA22130.12185291312707723No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA21740.11970548266528055No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT21710.11954029570668082No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG21620.11904473483088159No Hit
CCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTG21370.11766817684255039No Hit
TAATTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAATGAAAT21330.11744792756441741No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG21310.11733780292535093No Hit
ACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTG21300.11728274060581767No Hit
GTGTTGAGAATGGAGCGATTTCTCTTGGGAATCCATGAATGTGTGGGATT21150.11645680581281895No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG21010.1156859333393535No Hit
CTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCA20860.11485999854635477No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT20830.11469481158775502No Hit
GGTTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGAAGCAGAC20680.11386887679475631No Hit
GTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTG20600.11342837823849032No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG20460.11265750576502487No Hit
TTCTTCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTC20410.11238219416735862No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT19960.10990438978836248No Hit
GCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCG19670.10830758252189827No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA19580.10781202164609906No Hit
GCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGGATTAGAAATC19480.10726139845076657No Hit
GGGCTATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTT19450.10709621149216683No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG18910.10412284623737146No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT18900.1040677839178382No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC18900.1040677839178382No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT18890.10401272159830496No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG18820.10362728536157223No Hit
ATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGG18790.10346209840297249No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT18690.10291147520764No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC18470.10170010417790856No Hit
CCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACA18350.10103935634350959No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG18330.10092923170444308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG100.007046073145.310881
GCAGGTA22800.0116.83372
CCGACGC2100.0113.91194145
CAGGTAC23850.0111.8987963
GGTACTG24050.0110.922415
GTACTGA26750.099.72656
GCGAAAG3950.097.3903052
AGTAGAA22000.095.4428251
AGCGAAA4050.095.079951
CGAAAGC4150.092.6176453
GGTCAAA4400.085.865521
AGGTACT33000.083.921234
AGCAGGT32450.082.171181
ACTGATC38900.074.5392468
TAGAAAC30050.069.9873353
AAATATA5500.069.8553545
TACTGAT39650.068.010257
GACAGTA34650.067.154697
TTTGGAC35050.066.003743
TTTTTGG35400.065.4721
Produced by FastQC (version 0.11.5)