FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284170
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA144761.1272650817259398No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG129151.0057079670137132No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC121130.9432551764953239No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG112120.8730931262994774No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT74790.582399526542436No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG64700.5038273748802728No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG58880.45850627253401033No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT56970.4436328523482093No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA55350.43101770014873414No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA51440.4005700179882726No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA47290.3682534243908517No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG45940.35774079755795574No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT45700.3558718861209964No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG44500.34652732893620003No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA42180.32846118504559363No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG39140.3047883068441094No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA36760.28625493509426325No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAAACACTAGCCAAAGGG36420.2836073105585709No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA33430.2603237889064532No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA33020.25713106520164775No Hit
TCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGC32140.25027838993279705No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA31450.2449052695515391No Hit
CTACTAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCA30860.2403108622690142No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC30650.23867556476167484No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG30130.2346262566482631No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG29970.23338031569029022No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC29050.22621615518194632No Hit
TTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTA27790.2164043701379101No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG27680.21554778572930375No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC27310.21266654726399153No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG26870.20924020962956616No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG26420.20573600068526754No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA26230.20425644579767474No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT26200.20402283186805484No Hit
GGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCT25930.2019203065014757No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG25580.19919481065591005No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGC25550.19896119672629015No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA25490.1984939688670503No Hit
CATCAATACCAGAAGTGCTGCACTCTATTCGAGAAATTCTTCCCTAGCAG24880.1937438189647788No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG24430.19023961002048015No Hit
ACAAACACAGAGACCGGAGCATGCACAAACAGATTGTGTATTGGAAGCAA23850.18572307404782856No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG23840.18564520273795526No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA23670.1843213904701091No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT23570.18354267737137606No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT23540.18330906344175615No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA23380.1820631224837833No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG22990.17902614139872447No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA22900.17832529960986473No Hit
GGTTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG22860.17801381437037153No Hit
CTTCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGG22820.1777023291308783No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATACCAGAAGTGCTG22410.17450960542607288No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT22120.17225133743974708No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA22100.17209559482000047No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT21500.16742331622760226No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG21490.16734544491772896No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG21270.16563227610051628No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG21070.16407484990305024No Hit
TAATTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAATGAAAT21010.1636076220438104No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA20870.16251742370558417No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA20830.16220593846609094No Hit
TCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTA20560.16010341309951173No Hit
ACAAAACACTAGCCAAAGGGGAATTCTTGAGGATGAACAAATGTACCAGA20460.1593247000007787No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA20430.1590910860711588No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA20010.15582049105648005No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA19980.15558687712686015No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG19900.1549639066478737No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG19480.15169331163319497No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT19450.15145969770357506No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT19380.15091459853446193No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA19320.15044737067522213No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG18780.14624231994206374No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA18750.14600870601244384No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG18180.14157004134966555No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA18040.1404798430114393No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG18020.14032410039169269No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT17890.13931177336333975No Hit
GCTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCTAGTGTTGAGAATG17580.13689776275726734No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA17510.13635266358815423No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA16930.13183612761550262No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG16910.13168038499575602No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT16530.12872127522057047No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC16400.12770894819221754No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT16280.1267744924737379No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA16240.12646300723424467No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC16230.1263851359243714No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG16070.12513919496639853No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT16060.12506132365652523No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG15930.12404899662817229No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT15860.12350389745905915No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC15840.12334815483931254No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT15830.12327028352943925No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT15700.12225795650108631No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT15540.12101201554311344No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA15490.12062265899374693No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC15450.12031117375425372No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC15440.12023330244438042No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT15380.11976607458514059No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG15320.11929884672590078No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG15240.11867587624691436No Hit
GATCTTAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA15190.11828651969754785No Hit
GTGTTGAGAATGGAGCGATTTCTCTTGGGAATCCATGAATGTGTGGGATT15030.11704057873957498No Hit
GAAAAGACAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAA14900.11602825171122204No Hit
GTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTG14890.11595038040134874No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC14700.11447082551375597No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT14680.11431508289400935No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG14650.11408146896438945No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT14430.11236830014717678No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG14430.11236830014717678No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG14330.11158958704844374No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT14310.11143384442869715No Hit
GGTTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGAAGCAGAC14240.11088874525958402No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA14150.11018790347072428No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC13990.10894196251275143No Hit
CCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTG13860.10792963548439849No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC13730.10691730845604552No Hit
GTGTTTTGTTGTCCACCTCCCTTTTTCTGAATATTGATGTGTTCTGTTGA13660.10637220928693242No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG13650.1062943379770591No Hit
GGGCTATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTT13440.10465904046971974No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC13270.10333522820187357No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC13190.10271225772288715No Hit
GTATTCTCATTCCTTTCAGGGATCATCTCCATTATTCTTTTGTCAGCTGT13160.10247864379326725No Hit
CTATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGAAT12980.1010769602155478No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA12930.10068760366618128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA16250.0114.817362
AGTAGAA16600.0109.862411
GGTACTG17100.0109.37685
CAGGTAC17250.0107.631283
GTACTGA18500.0101.09576
CCGACGC1300.083.64358145
AGCAGGT22400.083.36211
GGTCAAA1200.078.712851
TAGAAAC23500.077.771483
AGAAACA23550.077.579164
ACTGATC26800.073.573068
AGGTACT26800.072.4995654
ACAAGGC17650.070.642898
AGCGAAA3050.069.084661
TCTGACC851.6916601E-1068.22692145
CTGCCGC2450.068.05287145
ATTACAC5250.066.3085563
TACTGAT29000.065.491967
CTTATAA2000.065.242145
GCGAAAG3250.064.780222
Produced by FastQC (version 0.11.5)