FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536969
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC60870.396039217446806No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG56710.36897295911628664No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG49090.31939486092432573No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT48980.3186791665934707No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG38110.2479555540807915No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG37540.24424695618454245No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC29740.19349772181481864No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA28590.18601546290133372No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC27580.17944408768166437No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC27110.17638612099528359No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA26730.17391372239778422No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA26670.17352334367186326No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT25820.1679929783879831No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG23970.1559563010054204No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG22660.14743303215614628No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC22330.14528594916358106No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG20690.1346155973217417No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA19280.12544169726259932No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT18200.11841488019602217No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT18070.11756905962319343No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA18060.11750399650220661No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC17460.11360020924299709No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA17430.11340501988003661No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG17410.11327489363806298No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC17180.11177844185536598No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC17050.11093262128253724No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA16790.10924098013687979No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT16490.10728908650727503No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG16130.10494681415174932No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG16060.10449137230484154No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG15800.10279973115918407No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT15640.10175872122339488No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA15380.10006708007773743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA8500.0110.16512
GACTTAA203.8690315E-4108.731804145
CCGACGC1600.0104.20131145
TCTGACC850.0102.335815145
GGTACTG9550.097.928295
CAGGTAC9700.095.688343
GTACTGA10550.090.011566
AGTAGAA5850.088.183271
AGGTACT12700.075.927314
AGCGTGC300.001937226572.48787145
GACGACA300.001937226572.48787145
GACAGTA10200.068.934547
CTGCCGC2300.066.18458145
CTTATAA2050.060.11189145
AACACTG3300.059.315975
TTTGGAC12300.058.3647773
TAACTAT6100.055.9824331
GTAGATG400.00602206754.442062
AGAAACA10050.052.6717263
CGCAATT450.00964648948.325245145
Produced by FastQC (version 0.11.5)