FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317517
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG70460.3040322897307765No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT45220.195122624774705No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA31470.13579188415877858No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT26910.11611565308906041No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT26870.115943054570905No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG26520.11443281753704504No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG23440.10114273163907753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC8300.049.8051723
TGATCCG7800.048.3248869
GTCATAG9200.041.0259742
TATAGCG3800.036.292212
GGATAGC8500.034.970515
CTATTAG34300.034.7298471
TAGAGGT33800.034.0990688
TAGTAGA33900.034.0043455
TATTAGT35450.033.579252
GCGAAAG12500.033.0984952
TAACTAT6700.032.5236741
GACAGTA9600.032.468287
ACTATAC9300.031.1928233
CTGCCGC5700.030.522293145
TATACTG21850.029.8626335
TATACTA7500.028.9999355
AGAGGTC40450.028.4931649
GCAGGTA7150.028.4246672
GTAGAGG41100.027.8661757
TTAGTAG45050.027.360794
Produced by FastQC (version 0.11.5)