FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2232_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2232_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276080
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT25860.20265187135602783No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG15470.12123064384678077No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG14350.11245376465425364No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG13390.10493072534637327No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG13130.10289323553382232No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT12940.10140430067080435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5300.041.0452583
TGATCCG5650.037.2099
CAGGTAC5700.036.8927233
TAACTAT4850.035.9377981
GGTACTG5500.035.5904585
TATAGCG3100.035.1104662
TATACTA5300.034.1976785
CGCTATA2404.3655746E-1133.2574162
GCGAAAG6800.033.079572
GTCATAG6600.032.982562
GACGTTC3750.032.8636748
GCAGGTA6000.032.6527372
TGACGCG900.003629526432.22055145
GTACTGA6250.031.3171466
CACGACG4000.030.8121075
CATAGTG6900.030.471824
ACTATAC6450.030.3543993
TATACTG6950.030.2514135
CTACTAG5900.029.4888389
ACGGTCG1000.00607778128.9973588
Produced by FastQC (version 0.11.5)