FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642302
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC55140.20868167226910472No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG42530.16095813423295294No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA40290.1524806778331924No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC39250.14854471593330362No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT35910.13590422290866072No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT31660.1198197632216151No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT31180.11800316542166642No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA29400.11126661524685673No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG28770.10888233063442408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA28000.044.0940971
ACATGGT29200.042.4542665
GTATACG1609.949872E-1040.8518871
CGAAGCG907.493004E-540.271199
GTATTAT29450.040.1968351
GCGAAAG4150.038.476232
GTGATCG2350.037.0152249
TATTATT28550.035.082532
GGTCCTA41450.034.451549
GACATGG35850.034.1780054
GTACTGC8800.032.955394
ATGGTCC43950.032.326917
CATGGTC38500.031.8206776
TGGTCCT46100.030.8192548
TATAATA15800.030.7776662
TATACTG10700.030.4885525
CCGACTA3201.8189894E-1229.4538824
TGACATG43400.029.2434793
ATCTATG15500.028.1131251
AGCGAAA5700.028.0309241
Produced by FastQC (version 0.11.5)