FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775597
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC37780.2127735066008785No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT33370.18793678971072827No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA28860.16253688196139102No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA27000.15206153198051134No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC23770.1338704672287687No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG22650.1275627296058734No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG22390.126098433371987No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC21810.12283192638870195No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG21420.12063548203787233No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT21120.11894590946031108No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA20700.11658050785172537No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT20590.11596099790661958No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT19640.11061068474434232No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA19340.10892111216678109No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA19300.10869583582310625No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA18600.1047534998087967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA23000.058.7665751
ACATGGT23450.057.1965645
GACATGG28050.047.5609444
AGTCCGC1307.368726E-944.606903145
GGTCCTA34750.043.804629
ATGGTCC37200.041.8950967
TGGTCCT36950.041.392678
CATGGTC32650.041.298486
CAGTGCA16250.040.2028162
AGTGCAG16800.038.418833
TGACATG37200.037.430163
GTGCAGC17250.037.4081654
GCAGTGC17750.036.845921
CTGCCGC5550.033.957508145
TAGTCGG1501.1362354E-633.82698
TACTGCG1551.4657762E-632.7421655
TTGACAT41300.032.691152
CTACATC7950.031.9274523
GGTTATA6700.031.453461
CAGCAAC15500.031.3336357
Produced by FastQC (version 0.11.5)