FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309103
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT30690.23443533472920006No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23790.1817274882113936No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT22830.17439422260891618No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG22050.16843594430690328No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG21020.16056796142091187No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT19940.15231803761812476No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA17920.13688762457957854No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17630.13467236726216347No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT16490.12596411435922156No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA15920.12160998790775057No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT15790.12061694152408177No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG14430.11022814858723873No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC14220.10862399673669682No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA13700.10465181120202154No Hit
AGATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTAT13480.10297127116812045No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA13210.10090879021742369No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG350.003568659862.13446145
GCCGACC1450.054.992565145
ACATGGT11350.053.016535
GTTGACA11750.051.3116761
GACATGG14650.040.580924
GGTCCTA16950.038.9180459
ATGGTCC17600.037.8926057
CATGGTC16150.036.806176
ACTCCGA2201.4551915E-1136.3200071
AGCGAAA2650.035.6347271
TGGTCCT19050.035.0083928
GCAGTGC10050.034.693741
CTTATAT6200.033.9767841
AGTCCGC1301.6567017E-533.457016145
GTGCAGC10250.033.2444884
GCGTTAA2404.3655746E-1133.2246789
CTGCCGC4700.032.38924145
CAGTGCA10350.032.258532
TGACATG18900.032.2363363
GCTACTA3850.032.008669
Produced by FastQC (version 0.11.5)