FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383419
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG26520.1916989718949935No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC25210.1822296787885666No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT23860.17247124696133276No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21100.1525206752256547No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20930.15129183566222526No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG20540.14847273313435772No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC19920.14399108296185031No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA19600.14167797319539488No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT19480.1408105570329741No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG18430.13322066561179224No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG17910.12946186224130218No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT17870.12917272352049525No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA17840.12895586947989005No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA17320.12519706610939996No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA16950.1225225329419359No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA16660.12042627721608565No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG16590.11992028445467354No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT15470.11182440027207954No Hit
AGATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTAT15130.10936672114522064No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA14750.10661990329755482No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT14150.10228282248545091No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA14080.10177682972403877No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG203.8658094E-4108.753994
GTCGTCG553.221725E-452.719673145
GTTGACA16200.049.7550851
ACATGGT16800.048.3281175
CAGTGCA11050.042.6918332
GCTACTA4300.042.1450929
GGTCCTA25200.041.1349459
GCCGACC1252.723027E-740.59415145
GACATGG20050.040.5002374
AGTGCAG12350.039.9277083
ATCTATG10250.039.672881
GTTACGT2057.2759576E-1238.964431
GTACTGC5650.038.4969864
GTATTAT15850.038.4839441
GGTTATA4750.038.21871
TGGTCCT27500.037.694578
ATGGTCC27650.037.4900787
GCAGTGC13250.037.266841
CATGGTC22050.036.821426
GTGCAGC12800.036.8177574
Produced by FastQC (version 0.11.5)