FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676474
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT33500.19982415474382545No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT24740.14757162950335048No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG24620.14685584148635766No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT21800.13003482308702669No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG19480.1161962547584991No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA17890.10671206353334438No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17160.10235768642997146No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG553.222635E-452.717316145
AGCGAAT604.9382873E-448.348723
CTAGTAC1151.4098259E-744.1444853
GTTACGT1859.458745E-1139.262591
GCGAATT800.002036509336.2518044
GGATAGC2850.035.611565
TATACTG9050.035.2461665
GTATATC3050.033.341021
TTACGTG2257.858034E-1032.252692
CCGACTA4300.032.036484
GTATTAT22450.032.0309261
GACTTAA8000.030.8702131
GTTATGT9000.030.6684441
AGGCGCG2151.8153514E-830.3431077
GATATGT7300.028.8553141
AGCGAAA3850.028.2996581
GTCGGCT1804.708276E-628.189966
ACTGGTT7300.027.8062865
TCGACTG1050.007717022727.6138349
TAGTCGG2903.965397E-1027.4948088
Produced by FastQC (version 0.11.5)