FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579104
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21560.13653312258090664No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20040.12690741078485016No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG19220.12171459257908283No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC17850.113038786552374No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA16250.10290645834599875No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG350.003570346662.127644145
CGAAGCG502.0130366E-457.9858069
CGACGAA604.951933E-448.3215145
GTTACGT2200.046.231931
GTACTAG1002.6964863E-643.590111
TTACGTG2350.043.2507632
GACGACT1851.8189894E-1243.1112064
GTTGACA14900.038.5192221
ACATGGT14900.038.4387055
AGCGAAA3250.038.0016331
GGTTATA4300.037.169861
AGACGAC2151.2732926E-1137.110973
TATTGGG8150.036.5224462
GTCGTCT1001.3938197E-436.241127145
ACGACTC2404.3655746E-1133.2283975
ACGTGTT3100.032.744294
CCGAGGT5050.031.5864284
ACTCCGA4850.031.456781
GTAACGG4400.031.3716681
GCTACTA3300.030.7500489
Produced by FastQC (version 0.11.5)