FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864495
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT116790.6263894512991453No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT105230.5643887486960276No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC91830.49251942214916106No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG86320.46296718414369575No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA79680.4273543238249499No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT73590.3946913239241725No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA73510.39426225331792253No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC73420.39377954888589134No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT71110.3813901351304241No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA65160.34947800879058405No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC64360.3451873027280845No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG59190.3174586147991815No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA58850.31563506472261926No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG54180.29058806808277843No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG53460.2867264326265289No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG52310.2805585426616859No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT47120.25272258708122036No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT45420.24360483669840896No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC44720.2398504688937219No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC43040.23083998616247295No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA42870.22992821112419182No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT42200.2263347447968485No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC41900.22472573002341117No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG39870.2138380633898187No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT39770.21330172513200624No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT39460.2116390765327877No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG39300.2107809353202878No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT36980.19833788773903926No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA36930.19806971861013306No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGCTC36850.19764064800388306No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA35700.19147275803904007No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT35670.19131185656169633No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC35040.18793292553747798No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG34850.18691388284763436No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG34840.18686024902185308No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT34610.1856266710288845No Hit
ACATACGCTTATACGTTTAGACATCCAGATAGCTGAAGATAACCAGAAAA33700.18074599288279133No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT33160.17784976629060417No Hit
GTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACA32510.17436356761482333No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG31780.1704482983327925No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG31510.16900018503669895No Hit
CTAATAACCATTTTCTCTCCTTTTCGTCATTCTTATATTCTAACGTAGCC30910.16578215548982433No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG30670.16449494367107448No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG30130.16159871707888732No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG29860.16015060378279372No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA29190.1565571374554504No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT28830.15462631972732563No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC27990.15012107836170116No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC27880.14953110627810748No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG27790.1490484018460763No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT27640.14824389445935762No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA27530.14765392237576394No Hit
GCCTGATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAA27320.14652761203435782No Hit
CTACATACGCTTATACGTTTAGACATCCAGATAGCTGAAGATAACCAGAA26840.1439531883968581No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG26390.14153966623670217No Hit
GCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGG26050.13971611616013987No Hit
ACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCT25560.13708805869685892No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC25530.1369271572195152No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG25280.1355863115749841No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC25210.1352108747945154No Hit
TTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCA24950.13381639532420309No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT24750.1327437188085782No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA24510.13145650698982836No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC24450.13113470403514088No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG24330.13049109812576595No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT24230.12995475986795352No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG24160.1295793230874848No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG24120.12936478778435984No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA23880.12807757596561No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA23850.12791667448826627No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC23730.12727306857889134No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG23630.1267367303210789No Hit
TCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTT23490.12598585676014148No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT23360.1252886170249853No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT23350.1252349831992041No Hit
CGCTTATACGTTTAGACATCCAGATAGCTGAAGATAACCAGAAAATTTAC23310.1250204478960791No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT23140.12410867285779796No Hit
GGTTAATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAAC22580.1211051786140483No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC22550.12094427713670458No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA22530.12083700948514209No Hit
AAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATA22490.12062247418201712No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT22460.12046157270467338No Hit
ATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGC22070.11836985349920488No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG22010.11804805054451742No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC21260.11402551361092414No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT21250.1139718797851429No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC21230.11386461213358041No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA21060.11295283709529925No Hit
AACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTT21040.11284556944373679No Hit
GGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTT20260.10866213103279977No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT20190.10828669425233105No Hit
CCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCG20180.10823306042654982No Hit
TTCTTATATTCTAACGTAGCCTTTTCCTTGAAACTTTCTCACCTTCAACA20170.10817942660076858No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT20090.10775035599451863No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGCGCGGCTGCGGCGTGG20060.10758945451717489No Hit
GGTTAAGGATAAGCACCTTTAGCGGACGAATTTCCTGACCAGACGCGCGA19990.10721401773670619No Hit
CTTATATTCTAACGTAGCCTTTTCCTTGAAACTTTCTCACCTTCAACATG19980.10716038391092494No Hit
GCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATC19730.10581953826639386No Hit
AATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATG19590.10506866470545644No Hit
GTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCT19410.10410325584139404No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCAC19410.10410325584139404No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT19170.1028160440226442No Hit
TCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT18860.10115339542342565No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC18740.10050978951405072No Hit
TAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAG18650.10002708508201953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAG19650.074.9643862
CATAGTG20250.073.748954
GCGAAAG7850.062.858032
AGTGATC26050.056.492447
CGAAAGC9700.051.5858763
TGATCCG29750.049.9538429
ATAGTGA32000.048.0285575
CGCGCCA26100.047.4960679
AGCGAAA10450.047.2847141
TAGTGAT32500.047.065336
GTTAAGG6250.046.440992
TAAGACG23800.045.6907164
GTGATCC32700.045.6689958
ACGCGCC27900.044.1719678
ATAAGAC24700.043.7464453
GTATTAT9650.042.1685681
TAAGGAT7000.041.426254
GACGGAT6700.041.1159937
AAGACGC26100.041.108815
GGACGGA6650.040.3350036
Produced by FastQC (version 0.11.5)