FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481649
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC51570.2078053745715047No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT27000.10879862543010715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1951.70985E-1037.17549
GCGAAAG5450.030.6238172
CTGCCGC10250.028.99798145
GTTGACA23650.027.9417571
TATACCG4950.027.8263455
TACGTCG1050.00771323527.6171265
GTATTAT22650.027.2517361
ACATGGT23300.027.068935
CGAAAGC6450.025.859253
GAATAAC19750.025.3702891
ATACCGA5450.025.2724536
GCAGTGC24200.025.2062021
AGCGAAA6750.024.7438641
TACTGCG2351.3325753E-624.679135
TACCGAG5700.024.1640137
GCGTTAA2401.6012054E-624.1640119
GTATAGG9200.023.6797351
AGTCCGC2802.3470966E-723.30195145
GGTCCTA40550.023.0615849
AGTGCAG26150.023.017293
Produced by FastQC (version 0.11.5)