FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2231_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2231_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595182
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC26760.16775515270357866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4850.037.410782
CGAAAGC5750.032.7926863
AGCGAAA6400.028.3708321
TATACTG8950.028.3508955
CTGCCGC6150.028.290737145
GAATAAC11650.028.0542491
GCGCATT1050.007712389327.617159
GCCGACC3750.027.064806145
GTTGACA13950.026.552661
ATAACAG14350.024.2583273
TCGCCAT5400.024.165005145
ACATGGT15100.024.0057265
GTGTTCC19900.023.314989
ATAATAC9700.023.1772273
TAATACA12250.023.0814934
CTATAGG4205.0931703E-1122.4805091
GTTACCG3554.132744E-922.4632439
GTACCGA4006.91216E-1021.7485077
TACATCG5400.021.4806775
GTATTCG1700.003088079421.3300883
Produced by FastQC (version 0.11.5)