FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686794
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG1656.033224E-835.143429
AGCGAAA6150.034.2440871
CGAAAGC6600.032.968463
GCGAAAG6700.031.4124812
TGATCCG1652.392002E-630.7504949
ACGCGCC2553.0340743E-928.4248288
TAGTACT6000.026.587464
AACGCGC1658.4351355E-526.3575677
CGCGCCA2808.22547E-925.8868969
AAACGCG1701.03311104E-425.5831036
GGGTTGA14000.023.8166526
GCCGACC1550.001811113723.382406145
GATAGCG1600.002178302722.651707145
ATCGTGC2903.296136E-722.4948228
GTGATCG2303.149266E-522.0601379
GATATAT10450.021.5430971
TACACCG2053.6684447E-421.2190285
GTTATAC6550.021.0394133
TCTATGA10700.021.0260162
CCGTATA1800.004283024520.1593512
Produced by FastQC (version 0.11.5)