FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564021
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17740.11342558699659405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1157.144792E-637.8264735
GCGAAAG5200.033.503662
GCCGACC2051.14159775E-831.823486145
AGCGAAA5050.031.6573581
ACGCGCC1153.1662814E-431.516018
CGAAAGC5500.030.3281883
GAATAAC10000.029.0672151
ATACCGA1050.007712986327.616686
CTGCCGC7900.027.526644145
AATACCG1409.996524E-425.8931225
ATACCGC2301.1046068E-625.2152276
GTATAAG5550.020.9493431
TAGTACT5103.274181E-1119.9027984
ATAACAG15600.019.0607763
GTCCTAT16350.018.6670191
ATTATAC7050.018.5167483
GTATTAT13550.018.2340451
TACGTAC2000.00790297318.130983
GTACTGC3602.6207472E-618.1234466
GCACCGT3302.515214E-517.574256
Produced by FastQC (version 0.11.5)