FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793854
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6200.035.1599121
TATACGC850.002730637634.1479842
GCGAAAG7700.028.2718722
CGCTTAT1100.00968033726.359865145
CGAAAGC8850.024.5830353
GTATATC4650.023.439941
ACCGTTC1902.1962379E-422.8914628
GCACCGT9350.020.9340026
ATATACG2155.04891E-420.2381273
TACACCG2908.257735E-619.9993695
GGACCGT2556.9660935E-519.9002236
TAGTACC8400.019.8522244
AGCACCG11400.019.0783465
GTATACG2307.845334E-418.9557761
CACCGTT2400.001060976318.1229137
GTGCTAT12150.017.941681
TCCGTAC2050.00911469417.6877923
GTACCGG2901.8344763E-417.5395411
GTATATA10600.017.1376911
GTCCTAT13650.017.034741
Produced by FastQC (version 0.11.5)