FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318428
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5300.030.139991
GCGAAAG5750.027.7812081
GCCGACC2756.7811925E-926.359568145
TAGTACC5001.8189894E-1221.7515954
TCAGGCG2704.452584E-621.4781679
GTATAAG4109.222276E-1021.25171
GTCCTAT11700.020.4797361
TATAAGA7550.020.1838862
GTATATA9150.019.838791
TAGTACT4803.1468517E-1019.6368544
TATACTG6300.019.5619565
TATACAT9150.019.01495
CGAAAGC8750.018.2451322
ACCGCTC2050.00913601917.6801998
GTACCTA7850.017.5450616
TATATAC8700.017.5079233
ATACAGC8200.016.7961886
ATTATGC3905.590491E-616.7383443
GTACAGT5252.0228981E-816.568876
TAATACA15800.016.5201994
Produced by FastQC (version 0.11.5)