FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874269
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5650.033.4397281
GCGAAAG6200.030.4407352
CGAAAGC6900.028.3833333
GATAGCG4450.027.691591145
TATACTG9300.021.0479265
GTTACGT1800.004251417720.1853031
GCCGACC2908.2688E-619.99636145
GTTATGT12050.019.9005321
CCGACTA4502.9958755E-919.334464
GATATAT11800.019.0905081
GCCGAGT3101.4701969E-518.7062748
GTATTAT24900.018.385651
TACGGAG2400.00106033718.1246015
TAGTACC10300.017.5981124
CTTATAT10450.017.3844721
CTGCCGC6857.2759576E-1216.931225145
GTATATC6053.6743586E-1016.8155271
GACTTAA10050.016.6302811
GACGATG3504.1711995E-516.568413145
ATAGGGC5854.3582986E-916.118933
Produced by FastQC (version 0.11.5)