FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703483
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC8950.026.7321034
CACGCCG1100.00967438226.363125
AGCGAAA5500.025.089891
GTATTAG6450.024.7725561
GCGAAAG5700.024.1874922
GTATAAG5200.023.7439771
ACGCCGA1851.8323754E-423.5116736
CTGCCGC8300.022.70773145
GCCGACC1952.6182178E-422.304636145
CTATACT5700.021.6230164
TATACTG7800.021.3777875
GTCCTAT16000.021.3346611
CGAAAGC7200.020.1449993
GTCCTAG9400.019.3161241
TCCTATG18800.019.2985322
GTACCTA9900.019.0389126
GAATAAC12600.019.0217841
CTAGTAC3852.3798748E-718.8368823
CGCCGAG2701.0806286E-418.7942717
TATTAGG6503.6379788E-1217.8615322
Produced by FastQC (version 0.11.5)