FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132673
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5750.053.001142
AGCGAAA6400.049.923241
CGAAAGC7800.039.046613
GACGACA1701.8353603E-938.37597145
CCGACGC6400.035.111347145
CTGCCGC9000.028.995176145
GTATTAG9350.028.7355941
AGCGTAT1450.001227921724.9964286
GTATAAT7400.024.5323051
GTATTAT25400.023.7287271
CTTCGAC2502.2770982E-623.2048443
CAGCGCG1902.1967749E-422.8909269
TATAATA18800.021.227982
TATACTG12400.020.4627045
GAATAAC13200.020.3543781
GAGCGAA2908.143754E-620.0318971
GTCCTAT18200.019.9493471
GTATATA11500.018.9432051
GTATTAC8700.018.3625721
GTGTAGG11100.018.3174551
Produced by FastQC (version 0.11.5)