FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535721
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC19500.12697618903433633No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC400.006042677454.3950583
CCGACGC3150.050.62886145
GAATAAC8200.034.5416831
CTGCCGC6300.034.51968145
ACCGCGC850.002744050234.1135648
AGCGAAA4600.033.1553961
GTATATA5000.029.050441
GCGAAAG5450.027.9615692
GTCCTAT15500.023.8963321
GTTATAC4002.7284841E-1123.5711923
GTATTAG7100.023.5267681
CGAAAGC6500.023.4317193
ATACCGT1600.00217595522.6557524
TATTAGG4950.021.9899792
GACCGCG1750.003662149220.7118077
TCCTATG18250.020.6766032
GTGATCG2908.262756E-619.9976069
GTTCTAT21350.019.3896391
TATGATG20150.019.0678165
CCTAATA4954.6929927E-1019.0579812
Produced by FastQC (version 0.11.5)