FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888383
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5450.039.9442672
CCGACGC3200.038.51159145
AGCGAAA5750.037.879291
GTATACG1905.358743E-934.3904081
CGAAAGC7200.031.2219263
TACACCG2603.7234713E-927.8853845
CGACGAA1050.007714100627.616154145
TATACGG1658.420879E-526.3643655
ACCGTGT6500.025.6511578
GCACCGT6100.024.9570626
AGCACCG6350.023.977045
GTATATA12800.020.9865071
CACCGTG9100.020.7121147
GTTTACG2505.913785E-520.3285541
ACCGTGC1800.004311079620.1367788
GTATAAG4702.3283064E-1020.0813961
GTGATCG2205.910198E-419.7706579
GCCGACC1850.00505156619.59254145
TAATGCG2359.2024944E-418.5111485
GATATAT9250.018.0523831
Produced by FastQC (version 0.11.5)