FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544223
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGCG805.88303E-754.3653349
AGCGAAA6350.045.779761
GCGAAAG6350.043.4526822
CGAAAGC6350.042.261223
CCGACGC3050.035.6494145
GCGTTAA1450.001228017724.9955569
TACTGCG1751.2568457E-424.8551395
CTGCCGC8750.024.852724145
TGTACCG2051.2916633E-524.75415
GTATTAG5150.023.9899271
TAGTACT4952.1827873E-1120.504064
TAGTACC8800.019.7692159
GTCCTAT12450.018.0958751
GTACCTA8450.018.0151316
TATACTA3652.9894327E-617.875275
GCACCGT5802.0736479E-1017.4974566
GCATCCG2500.001395521117.3969087
GTACTAG4307.419494E-716.9012491
TATAGTA4307.4878153E-716.8864482
TAGGAGT8650.016.7621974
Produced by FastQC (version 0.11.5)