FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889911
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC25370.1342391255461236No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG450.00956509548.4294361
TATTGCG700.001057844641.420897
GCGAAAG5450.037.2921072
GCCGACC2252.0008883E-1135.43787145
AGCGAAA6050.033.6204381
GATAGCG2351.2660166E-930.845345145
CGAAAGC7000.029.0069033
ATTGCGT1050.007717139627.6139288
ACCGTGC2207.5040225E-726.3587478
TACACCG2651.3776844E-724.6226375
GTGATCG3301.7789716E-924.1621869
GTCCTAT25450.023.9768521
GACCGTC2151.8695573E-523.6002757
TCCTATG21350.022.7788752
TATAATA14300.021.8266852
GACGAAC2353.7167414E-521.591742145
GTATATA16200.021.5241951
ATAGCGC1700.003097129821.319576145
TATCGAT5251.8189894E-1220.7109936
GTATATC8000.019.9771441
Produced by FastQC (version 0.11.5)