FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2230_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2230_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550126
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15560.10037893693802956No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC651.0940088E-555.7685245
GCCGACC1002.7390051E-643.49103145
AGCGAAA4750.033.6469081
ACGTCCG900.003632508232.215589
GCGAAAG5350.031.2000392
CGAAAGC5350.031.1819133
ACTGTCG1402.751517E-531.0650228
GTATACG1804.6287532E-628.251511
GTATTAT15900.024.6724781
GATAGCG2151.8695768E-523.599783145
TAGCGTG1550.001811107623.3822759
GTATAAT7850.023.1359041
GTCCTAT13400.021.6855931
TCCTACG1700.003075299321.3452972
GTGATCG2404.3718166E-521.1414749
GTATAAG5550.020.9432031
TATAATA11300.020.5519312
TAGTACC6050.020.3736154
CTGCCGC5800.019.995874145
CTAGGGC7450.019.4716383
Produced by FastQC (version 0.11.5)