FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2044_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2044_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662694
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA3550.046.9640469
ACGCGCC3800.043.8743068
GACGCGC3450.039.9209187
TGATCCG3400.038.3759969
GCCGACC3400.038.375996145
AGACGCG4350.031.6642746
GCGAAAG5650.029.5492612
TAAGACG5200.027.8858664
CGAAAGC6100.026.1573663
AGCGAAA6250.024.4117551
CGACGAA2401.6014528E-624.162663145
CGCTTAT1550.001809785423.3853326
ACCGTGA8750.021.5392888
GTCATAG7150.021.319672
CATAGTG6500.021.1932564
GAATAAC13850.020.9831161
GAACCGT8000.020.8421766
TATAATA11950.019.4378992
AAGACGC7550.019.2038465
GTATATA9150.019.0567971
Produced by FastQC (version 0.11.5)