FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2044_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2044_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685406
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC953.144305E-853.4142157
GACGCGC3550.051.0497977
ACGCGCC4600.050.4268268
CGCGCCA4800.048.3257069
AGCGAAA4800.042.372991
GCGAAAG5400.040.3192672
AGACGCG5100.038.3797156
TGATCCG3650.037.7337729
TAAGACG6100.030.9022644
CGAAAGC7500.029.9743923
CTGCCGC7300.028.796827145
TACCGCG1050.007714746527.6155097
GTATTAG6350.026.3103371
AAACGCG1958.763818E-626.0238516
GTGTAAA8050.023.4611741
GAATAAC11250.022.5989281
ATAAGAC11050.022.313443
AAGACGC9250.021.1613355
GTATATA9750.020.860551
TATGCGA1800.0043077720.1392974
Produced by FastQC (version 0.11.5)