FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1805_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1805_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803606
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG10150.040.040072
AGCGAAA10800.037.663881
TAACCGC1102.4382582E-432.952265
CGAAAGC12600.032.8052863
GCCGACC4650.026.502594145
CGCTAGT1400.001000523125.890139145
GTGATCG2051.2927547E-524.7526159
CTGCCGC13400.022.180506145
AGCACCG13200.020.8697645
GTTATAC10600.020.5236683
GTCCTAT16050.020.3656481
GCACCGT12900.019.6684766
CGATAAC2959.580999E-619.65895145
TATAATA23700.018.6790312
GTGTAAG22400.018.159371
GTATAGA20850.017.767441
ACCGTTA2050.00913779817.6804398
TCCTATG21850.016.939152
AATACCG3052.746199E-416.6375966
CACCGTG17000.016.6300137
Produced by FastQC (version 0.11.5)