FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1805_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1805_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791470
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA12100.046.2272641
GCGAAAG13450.041.5448462
CGAACCG800.002038176636.24665
CGAAAGC15900.035.1187253
ACCGTAC850.002745047734.1120078
TGACGCG1753.7816171E-628.996243145
CCGGTAA3600.028.19079145
CCATCGA5100.025.5840039
CTGCCGC15200.022.89177145
TATACCG2003.106934E-421.7479615
GTATTAT22750.021.3937191
CGTTAAC1700.003096492321.320768145
CTATGCG6550.021.0311994
GTATATA16550.020.1907621
GCCGACC4503.0049705E-919.330828145
CCTATGC9850.017.6693333
GTATAAT13250.017.5439321
GTGTACA15100.017.3188251
GTATATG15350.017.0367581
GTCCTAT24100.016.5782431
Produced by FastQC (version 0.11.5)