FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1805_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1805_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790336
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG8600.037.1310162
AGCGAAA9200.035.5320631
CGAAAGC9600.034.7547573
ATCGATA1102.4390596E-432.9496048
CGCCGAT950.004731749730.5217427
CCGACGC1952.6126872E-729.739132145
CGTAGCG1000.00604540529.0297052
GCGCCGA1000.006078511528.9972746
TACACCG2903.965397E-1027.4981845
TATACTG11400.024.1650715
CCCTACG1902.1789489E-422.9181882
CTGCCGC6000.021.746742145
CCTACGG2053.657198E-421.2287833
TCTAACG2104.3032636E-420.7233373
GCACCGT8900.018.7341946
TAGTACC10450.018.0355749
ACACGAC2851.6357985E-417.804348145
GCCGACC2450.001218659217.752441145
AGCACCG9850.017.6638075
GTGTACA8650.017.6359521
Produced by FastQC (version 0.11.5)