FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1805_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1805_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817227
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC31220.17180022088599828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG4750.039.6750229
CCGACTA2951.4551915E-1129.4924
ACCGTAC1306.4189243E-427.93351
CCATATG21500.027.6805572
CGAAAGC8400.027.6240563
CATAGTG8700.026.6670974
GATAGCG3057.1304385E-1026.141487145
TATGCTG22100.025.5851275
CGGTAGA1450.00121436625.0438271
TATACTG9550.024.2902435
GATGCGC11200.023.9453478
GTACCGT2151.8689785E-523.6011356
TCCATAT23750.023.2406711
ACACCGT4505.456968E-1222.5521956
GTTAAGG8700.022.523922
GTATTAG6500.022.3467981
GATCGAT8900.021.9898957
TATACAG13250.021.8841345
ATGCTGC25250.021.8185316
TATAACG2353.714806E-521.5931225
Produced by FastQC (version 0.11.5)