FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1805_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1805_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085802
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11680.10757025682398816No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC750.001485031438.65824145
GCCGACC1001.3933124E-436.242104145
GTGATCG900.003632240232.2152029
ACGTCCG1255.160704E-428.9936839
TATACTG6400.028.3206635
GCGAAAG3700.027.4567992
ACTCCGA2156.029295E-727.0256251
TATAACG1358.082578E-426.8521845
CGAAAGC3800.026.714553
CTGCCGC4001.8189894E-1225.369473145
GTATTAG5250.024.9021821
AGCGAAA4101.8189894E-1224.8009571
CCGACTA2151.865084E-523.6060314
GCTACTA1952.6188017E-422.3028329
TAACGTC1650.002604294821.964917
AGTCCGC1700.003096874621.318884145
CCGAGGT3457.5013304E-821.015724
ATCTAAA15650.020.420321
GTATTAT10750.020.2692181
ATTATAC5204.3655746E-1119.5221713
Produced by FastQC (version 0.11.5)