FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1805_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1805_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380123
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20570.14904468659677433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3250.051.371752
CGAAAGC3550.046.994673
AGCGAAA4400.037.9836271
GTAGGCG1553.475907E-837.4144949
GCCGACC1854.1909516E-935.265686145
TATACCG950.004729239730.5245655
GTGATCG1703.0158608E-629.8490649
CCTCGCG1255.158764E-428.9962339
CTGCCGC4400.026.360212145
GTCCTAT12550.026.054941
ATAGCAC6050.021.5807363
GCACCGT4501.3278623E-1020.9424826
TATACTG9750.020.8193175
TCCTATG14650.019.8199372
GAATAAC10100.019.4251171
GTATAAG6101.8189894E-1219.0594961
TATGATG14500.017.9989665
GAATAGT8100.017.8995576
GTATATA9350.017.8746471
GTACTGC3703.4037075E-617.6334826
Produced by FastQC (version 0.11.5)