FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1803_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1803_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672496
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA400.00605216354.37015145
GCGAAAG3000.041.104122
CGAAAGC3350.036.8014453
TAACGGG1001.3868314E-436.268342
AGCGAAA3500.035.250461
TACACCG1551.4642319E-632.7414475
GTGATCG900.003628977632.219359
CACCGTT1352.1424717E-532.219357
GTATAAC1855.7662E-627.4606111
GTATTAG2401.5825735E-624.191491
ACCGTTC1550.001808814523.3850128
TATACTA3503.4960976E-922.7853765
TATACTG3551.01363185E-720.4222325
GTACTGG3208.379193E-720.4115721
CTGCCGC2505.9748752E-520.29819145
CTATACT3301.1370066E-619.7724324
GTATTAC3751.7865386E-719.3531931
GTTATAC2659.323626E-519.1563573
ACACCGT2659.350285E-519.1492356
GTATTAT8800.018.9683281
Produced by FastQC (version 0.11.5)