FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1803_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1803_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051415
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG9700.032.1708072
AGCGAAA10250.030.474311
CGAAAGC10850.030.08113
TATCGGT1450.00121986825.0246032
ACTACGC2053.6200075E-421.2611481
TATACTA9050.020.827885
GTATACG2505.888914E-520.339831
CTACGCA2155.025189E-420.2524682
CTGTCGA3552.2953463E-618.3772959
ATCGTGT2851.636105E-417.8041238
TAAATCG3302.5148596E-517.575045
GTTATAC9750.017.1093753
GTATTAT22550.017.0733451
CTAATAC7950.016.421633
TATGCCG2650.002060993616.4124269
GTATATA17150.015.672091
TATACTG15300.015.6366195
TATTATA20000.014.8771272
TATAGAC11750.014.8230842
TAGACAG12250.014.7953165
Produced by FastQC (version 0.11.5)