FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1803_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1803_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240103
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG9650.042.8769872
CCGACGC1755.2750693E-1141.423515145
AGCGAAA10450.037.535781
CGAAAGC11500.036.581983
GTGATCG3400.034.1134839
TATACTG16150.022.8934755
ATTACGC2053.6570602E-421.229243
TACACCG3106.254413E-721.0472285
GTTACGT2908.121728E-620.038261
GCCGACC3902.7387796E-718.587475145
GCGTAAC2000.00783228518.1596741
CTGCCGC10150.017.854963145
TCGCACC2050.00912804117.683534
GATAGCG3703.4065488E-617.632982145
GATATAT15850.017.4149551
GCGGACC2500.001395266317.3978778
GTATATC4658.969255E-817.1833461
GTTGACA13550.017.1545241
CGACTCC3403.251474E-517.0571236
GTATAAT10250.017.0080831
Produced by FastQC (version 0.11.5)