FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1803_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1803_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469131
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5350.047.480032
GCGATTA803.7377577E-545.307896145
AGCGAAA6350.040.0328671
TGACGCG750.001484354538.662735145
CGAAAGC7150.036.5184333
TATACCG1306.48439E-427.8846285
GATAGCG2104.9935625E-727.61624145
GCGGACC1100.00967782726.3609569
TCGACAC2001.0625361E-525.385383
TATACTG12750.025.019625
TTATACT13800.023.1175294
CCTTATA11250.021.9342652
GTTGACA8950.021.0995251
TACCGAC1750.003661690720.7121818
CTGCCGC5700.020.348808145
TGTACCG2505.976919E-520.300015
GTACCGA1800.004310551620.1368437
TATAGAC6900.019.9848652
GTATAGA14150.019.5051611
GTCTAAG5356.366463E-1119.006261
Produced by FastQC (version 0.11.5)