FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1803_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1803_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242019
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4100.056.6477622
AGCGAAA4600.050.5209431
CGAAAGC5350.042.0217973
GCCGACC2303.4928235E-828.366549145
CCTACGG1409.93187E-425.9214122
GTCTGCG1400.001000215925.8901049
GTGATCG1450.00122737124.9973419
GTATAGG5000.024.692111
TATACTG10200.023.4552785
CGACACT1550.001809006723.3864924
CTGCCGC4307.2759576E-1121.916273145
CCGACAA2704.4503886E-621.479198145
GTACTAC2104.3168679E-420.7129176
CCTATAC3953.1144737E-718.3598693
TATAATA13400.017.8741652
GTATAAG5902.582965E-1017.2327791
ATACAGC10550.017.1789596
TTATACT9350.017.0583844
ATAGGGC4109.003485E-615.9193513
GTACTGC4151.0135307E-515.7218516
Produced by FastQC (version 0.11.5)