FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1803_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1803_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726538
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG73210.42402773642978026No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC67880.3931567101332262No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA64550.3738695586196192No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT50250.29104485392154705No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG45000.2606371826163108No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG44150.25571403583355823No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA43670.25293390588565096No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC42720.24743156536375105No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG38020.22020945962382527No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG36410.21088444042355278No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC30570.17705952605734712No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT29830.17277349238765669No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG29720.1721363792745946No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC29460.17063047555281147No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA29040.16819786184839258No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC27630.16003123012641482No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA27450.1589886813959496No Hit
TGCTAGTACTACTTGAAAATCAAAAAACACTCGATGAGCATGATGCGAAC27350.1584094876568022No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT27200.15754069704808119No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG26520.15360217962187916No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC26500.1534863408740497No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT26020.15070621092614236No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA25670.14867903283912662No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT24570.14230790170850569No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG24500.14190246609110255No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC23940.13865898115187733No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC22980.1330987212560627No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA22850.13234576939517115No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG22740.13170865628210907No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA22680.13136114003862062No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT22670.1313032206647059No Hit
TTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTGT22660.13124530129079115No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT21910.126901348247186No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT21530.12470041203842602No Hit
GGCATATAATGCAGAATTGCTAGTACTACTTGAAAATCAAAAAACACTCG21460.12429497642102288No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC21450.12423705704710813No Hit
ATCCAATGGGAATCTAATTGCTCCATGGTATGGACACGTTCTTTCAGGAG20730.12006686212524718No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC20630.11948766838609981No Hit
ATCTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTG20390.11809760341214616No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC20300.11757632904691354No Hit
TGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATTG20010.11589666720338619No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC19570.11334821475113782No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT19400.11236358539458732No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC19230.11137895603803682No Hit
GTGCATGGAAACAATTCGGAACGGGACCTATAATAGGAGAAAGTATAGAG19180.11108935916846313No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT19140.1108576816728042No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT19120.11074184292497473No Hit
ACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAATCAAAAA19050.11033640730757158No Hit
CTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTA18650.10801963235098214No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC18620.10784587422923793No Hit
ACCAAATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTT18510.10720876111617583No Hit
ATTATAGGTCCCGTTCCGAATTGTTTCCATGCACTGATCATCACATTTAT18490.10709292236834636No Hit
CTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGATATTGTGG17780.10298064682040013No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA17700.10251729182908224No Hit
ATTCACCACTGTCCAGGCCATTGCTCGGTCTCTTCCTTCAATTATTTCAA17580.10182225934210541No Hit
ATATTGTGGAATGGCAATGTACTGTTTAAGCCACCTTTTTCAGTCTGACA17560.10170642059427594No Hit
GTCCAGGCCATTGCTCGGTCTCTTCCTTCAATTATTTCAAACCTGTGCTT17470.10118514622904332No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT17420.10089554935946964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA7250.091.082462
TACGGTA259.389798E-486.9956055
GGTACTG7700.085.677485
CAGGTAC8750.076.24683
GTACTGA9550.074.389846
AGGTACT9550.070.600574
CTGCCGC3800.064.85763145
GCGAAAG1102.5465852E-1159.371932
TAACTAT5000.058.099721
ACTATAC5250.056.6325873
AACTATA5150.056.3617022
GGATAGC14850.055.165565
CGCAATT553.2223048E-452.7185145
GACAGTA7250.048.9932677
TATACTA7300.048.7084772
TATGGAT17300.048.2373122
TATAGCG5050.045.9822242
GCGGTAT5300.043.7688376
TTTGGAC8650.041.0791853
TATTAGT19350.040.5017852
Produced by FastQC (version 0.11.5)