FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1802_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1802_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394206
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG450.009647964548.32314145
CGAAAGC7650.042.6579483
TGATCCG2100.041.4198349
CGACGAA750.00148512638.658512145
AGCGAAA8600.038.01121
GCGAAAG8600.037.9757082
AATAGCG800.002037924536.246255
GCCGACC1406.6230314E-736.24235145
CGATTAC850.002738923334.1263583
ATCGATA1957.010385E-933.4544838
GATCGAT2054.048543E-728.2867157
GTATAAT7850.027.7618941
TTACGAG1050.007699874727.6261023
TAATACA15000.023.6825834
TATAATA16600.023.1717932
TCTATAC11700.022.3133893
GTATTAG4409.458745E-1121.4628891
GTAGTAT4751.0913936E-1121.410751
CCGGTTA5151.8189894E-1221.1120519
ATAATAC15600.020.9188023
Produced by FastQC (version 0.11.5)