FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1802_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1802_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993914
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG12900.032.5952535
AGCGAAA10650.032.0531771
CGAAAGC11000.030.9850313
GCGAAAG11050.030.8649622
GTTACGT1652.35458E-630.8132081
GCCGACC2705.5788405E-926.847803145
CTATACT6250.022.0405454
CCGACTA3601.17721356E-720.1393874
GTGATCG2908.2670085E-619.9969849
GTAATAT8100.019.7269741
GTATTAT24850.019.582641
CGCGTTT2000.00792565318.122267145
CTGCCGC9000.017.719551145
GATATAT11350.017.277891
TAGACAG12400.016.9547885
TATTATC13500.016.6631872
GGACCGA4801.3182034E-716.6124956
TACTCCG2650.002058388216.41554
GATATCG3153.512303E-416.1086839
GTATACG2750.002604708515.8467911
Produced by FastQC (version 0.11.5)